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cd86  (Miltenyi Biotec)


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    Miltenyi Biotec cd86
    EZH2 inhibition reduced the inflammatory phenotype of macrophages during OA. Macrophage differentiation and M1-like polarization were induced from the THP-1 monocytic cell line ( a ) or from human bone marrow-derived cells isolated from OA patients ( b ) in the presence or absence of EPZ-6438. The expression of pro-inflammatory markers (IL-1β, IL-6 and CXCL1) was analyzed by RT-qPCR. Experiments using THP-1 cells were independently repeated at least three times. Experiments using human bone marrow-derived cells were performed using biological replicates from independent OA patients. Data are presented as mean ± SEM. *: p -value < 0.05; **: p -value < 0.01; ***: p -value < 0.001. Spleens from OA mice described in Fig. and treated with vehicle or EPZ-6438 were collected and analyzed by flow cytometry. Cells were stained for macrophage-associated markers ( c ). Samples were acquired using a FACSVerse flow cytometer and analyzed with FlowJo 7.6.5 software. Results are presented as median ± interquartile range (n = 8 mice). *: p -value < 0.05; **: p -value < 0.01
    Cd86, supplied by Miltenyi Biotec, used in various techniques. Bioz Stars score: 94/100, based on 21 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/mouse+antibody/CD86+Antibody%2C+anti-mouse%2C+REAfinity/pmc13694607-279-27-31
    Average 94 stars, based on 21 article reviews
    cd86 - by Bioz Stars, 2026-10
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    Images

    1) Product Images from "EZH2 coordinates inflammatory and pain-associated pathways across joint compartments in osteoarthritis"

    Article Title: EZH2 coordinates inflammatory and pain-associated pathways across joint compartments in osteoarthritis

    Journal: Molecular Biomedicine

    doi: 10.1186/s43556-026-00643-w

    EZH2 inhibition reduced the inflammatory phenotype of macrophages during OA. Macrophage differentiation and M1-like polarization were induced from the THP-1 monocytic cell line ( a ) or from human bone marrow-derived cells isolated from OA patients ( b ) in the presence or absence of EPZ-6438. The expression of pro-inflammatory markers (IL-1β, IL-6 and CXCL1) was analyzed by RT-qPCR. Experiments using THP-1 cells were independently repeated at least three times. Experiments using human bone marrow-derived cells were performed using biological replicates from independent OA patients. Data are presented as mean ± SEM. *: p -value < 0.05; **: p -value < 0.01; ***: p -value < 0.001. Spleens from OA mice described in Fig. and treated with vehicle or EPZ-6438 were collected and analyzed by flow cytometry. Cells were stained for macrophage-associated markers ( c ). Samples were acquired using a FACSVerse flow cytometer and analyzed with FlowJo 7.6.5 software. Results are presented as median ± interquartile range (n = 8 mice). *: p -value < 0.05; **: p -value < 0.01
    Figure Legend Snippet: EZH2 inhibition reduced the inflammatory phenotype of macrophages during OA. Macrophage differentiation and M1-like polarization were induced from the THP-1 monocytic cell line ( a ) or from human bone marrow-derived cells isolated from OA patients ( b ) in the presence or absence of EPZ-6438. The expression of pro-inflammatory markers (IL-1β, IL-6 and CXCL1) was analyzed by RT-qPCR. Experiments using THP-1 cells were independently repeated at least three times. Experiments using human bone marrow-derived cells were performed using biological replicates from independent OA patients. Data are presented as mean ± SEM. *: p -value < 0.05; **: p -value < 0.01; ***: p -value < 0.001. Spleens from OA mice described in Fig. and treated with vehicle or EPZ-6438 were collected and analyzed by flow cytometry. Cells were stained for macrophage-associated markers ( c ). Samples were acquired using a FACSVerse flow cytometer and analyzed with FlowJo 7.6.5 software. Results are presented as median ± interquartile range (n = 8 mice). *: p -value < 0.05; **: p -value < 0.01

    Techniques Used: Inhibition, Derivative Assay, Isolation, Expressing, Quantitative RT-PCR, Flow Cytometry, Staining, Software

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    other:

    Article Title: Ejection of damaged mitochondria and their removal by macrophages ensure efficient thermogenesis in brown adipose tissue
    Article Snippet: Anti-F4/80 MicroBeads UltraPure, mouse antibody, Miltenyi Biotec , Miltenyi Biotec , Cat# 130–110-443; RRID:AB_2858241.


    Article Title: HLA-DR + NK cells are mostly characterized by less mature phenotype and high functional activity
    Article Snippet: NK cell stimulation for functional assays To estimate functional responsiveness, NK cells were incubated for 20 h in culture medium, containing IL-2 (500 U mL −1 ), or IL-12 (10 ng mL −1 ; BD Biosciences) + IL-15 (10 ng mL −1 ; Sigma-Aldrich), or C1R cells + anti-CD20 antibody Rituximab (Roche, Switzerland).

    Article Title: Ejection of damaged mitochondria and their removal by macrophages ensure efficient thermogenesis in brown adipose tissue
    Article Snippet: CD11c-APC, mouse antibody, Miltenyi Biotec , Miltenyi Biotec , Cat# 130–102-800; RRID:AB_2660155.

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    Article Snippet: CD45 MicroBeads, mouse antibody , Miltenyi Biotec , Cat# 130-052-301; RRID:AB_2877061.

    Magnetic Beads:

    Article Title: MicroRNA-24 regulates macrophage behavior and retards atherosclerosis.
    Article Snippet: .. Alternatively, mouse monocytes were positively selected from PBMC preparations using magnetic beads conjugated with a mouse antibody for the monocyte/macrophage cell marker, CD11b (Miltenyi Biotec, Surrey, UK). .. After density gradient isolation, PBMC were re-suspended in 90l of Macs Rinsing buffer (PBS pH 7.2, 0.5% bovine serum albumin (BSA), 2mM EDTA) (Miltenyi Biotec, Surrey, UK) and 10l of rat anti-mouse CD11b MicroBeads (Miltenyi Biotec, Surrey, UK ) were added to each sample (per 107 total cells).

    Marker:

    Article Title: MicroRNA-24 regulates macrophage behavior and retards atherosclerosis.
    Article Snippet: .. Alternatively, mouse monocytes were positively selected from PBMC preparations using magnetic beads conjugated with a mouse antibody for the monocyte/macrophage cell marker, CD11b (Miltenyi Biotec, Surrey, UK). .. After density gradient isolation, PBMC were re-suspended in 90l of Macs Rinsing buffer (PBS pH 7.2, 0.5% bovine serum albumin (BSA), 2mM EDTA) (Miltenyi Biotec, Surrey, UK) and 10l of rat anti-mouse CD11b MicroBeads (Miltenyi Biotec, Surrey, UK ) were added to each sample (per 107 total cells).

    Negative Control:

    Article Title: HLA-DR + NK cells are mostly characterized by less mature phenotype and high functional activity
    Article Snippet: Next, cells were fixed and permeabilized with CytoFix/CytoPerm solutions (BD Biosciences), washed two times with CytoPerm/CytoWash solution and incubated for 30 min at 4°C with IFN-γ-FITC mAb (Miltenyi Biotec). .. Isotype-matched mouse antibody (Miltenyi Biotec) was used as a negative control for the staining. ..

    Staining:

    Article Title: HLA-DR + NK cells are mostly characterized by less mature phenotype and high functional activity
    Article Snippet: Next, cells were fixed and permeabilized with CytoFix/CytoPerm solutions (BD Biosciences), washed two times with CytoPerm/CytoWash solution and incubated for 30 min at 4°C with IFN-γ-FITC mAb (Miltenyi Biotec). .. Isotype-matched mouse antibody (Miltenyi Biotec) was used as a negative control for the staining. ..



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    Image Search Results


    EZH2 inhibition reduced the inflammatory phenotype of macrophages during OA. Macrophage differentiation and M1-like polarization were induced from the THP-1 monocytic cell line ( a ) or from human bone marrow-derived cells isolated from OA patients ( b ) in the presence or absence of EPZ-6438. The expression of pro-inflammatory markers (IL-1β, IL-6 and CXCL1) was analyzed by RT-qPCR. Experiments using THP-1 cells were independently repeated at least three times. Experiments using human bone marrow-derived cells were performed using biological replicates from independent OA patients. Data are presented as mean ± SEM. *: p -value < 0.05; **: p -value < 0.01; ***: p -value < 0.001. Spleens from OA mice described in Fig. and treated with vehicle or EPZ-6438 were collected and analyzed by flow cytometry. Cells were stained for macrophage-associated markers ( c ). Samples were acquired using a FACSVerse flow cytometer and analyzed with FlowJo 7.6.5 software. Results are presented as median ± interquartile range (n = 8 mice). *: p -value < 0.05; **: p -value < 0.01

    Journal: Molecular Biomedicine

    Article Title: EZH2 coordinates inflammatory and pain-associated pathways across joint compartments in osteoarthritis

    doi: 10.1186/s43556-026-00643-w

    Figure Lengend Snippet: EZH2 inhibition reduced the inflammatory phenotype of macrophages during OA. Macrophage differentiation and M1-like polarization were induced from the THP-1 monocytic cell line ( a ) or from human bone marrow-derived cells isolated from OA patients ( b ) in the presence or absence of EPZ-6438. The expression of pro-inflammatory markers (IL-1β, IL-6 and CXCL1) was analyzed by RT-qPCR. Experiments using THP-1 cells were independently repeated at least three times. Experiments using human bone marrow-derived cells were performed using biological replicates from independent OA patients. Data are presented as mean ± SEM. *: p -value < 0.05; **: p -value < 0.01; ***: p -value < 0.001. Spleens from OA mice described in Fig. and treated with vehicle or EPZ-6438 were collected and analyzed by flow cytometry. Cells were stained for macrophage-associated markers ( c ). Samples were acquired using a FACSVerse flow cytometer and analyzed with FlowJo 7.6.5 software. Results are presented as median ± interquartile range (n = 8 mice). *: p -value < 0.05; **: p -value < 0.01

    Article Snippet: The following antibodies were used for immune cell characterization: CD11b (REA592 VioBlue, 130–113–810, Miltenyi Biotec), F4/80 (REA126 PE-Vio770, 130–118–459, Miltenyi Biotec), CD80 (REA983 APC-Vio770, 130–116–463, Miltenyi Biotec), CD86 (REA1190 PE, 130–122–129, Miltenyi Biotec), MHC-II (REA813 FITC, 130–112–386, Miltenyi Biotec), CD38 (REA616 PerCP-Vio700, 130–109–260, Miltenyi Biotec), and iNOS (REA982 APC, 130–116–423, Miltenyi Biotec).

    Techniques: Inhibition, Derivative Assay, Isolation, Expressing, Quantitative RT-PCR, Flow Cytometry, Staining, Software